# Acquisition
Hippocampal neurons were imaged using Zeiss LSM780/880 confocal microscopes and 63X (high-resolution in-situ hybridization samples) and 40X (CaMKIIalpha protein, immunofluorescence samples) oil objectives (NA 1.4). Z-stacks spanning the entire volume of neurons were obtained.

# Channels
mRNA images with 4 channels
* Ch1 = DAPI (nucleus)
* Ch2 = n/a
* Ch3 = MAP2 (dendritic arbor)
* Ch4 = Camk2a mRNA in-situ (Panomics)

Protein images with 3 channels
* Ch1 = Camk2a antibody
* Ch2 = DAPI (nucleus)
* Ch3 = MAP2 (dendritic arbor)